The laboratory enables conducting experimental research focused on the human factor in Industry 4.0 and 5.0, considering the interaction with an advanced workstation equipped with a collaborative robot (cobot, UR10e; Universal Robots A/S©). These studies encompass variables related to the worker (e.g., well-being, satisfaction) and work performance, considering both adult and senior operators (> 50/55 years). Besides, the laboratory is equipped with various state-of-the-art wearable devices (e.g., several eye-tracking glasses for ocular behavior recording, Pupil Labs GmbH©; a portable amplifier and surface sensors for monitoring cardiac, electrodermal, and electromyographic activities, Thought Technology Ltd.©) to infer the level of stress and mental overload experienced by individuals during the execution of specific experimental tasks. Lastly, this laboratory incorporates an algorithm based on psychophysiological indices, capable of identifying stress and mental overload conditions and automatically implementing changes to the operating of the workstation and cobot.